miniaturized transmitter for telemetry-ecg recording model ta11cta-f40  (Data Sciences International)

Journal: Circulation. Arrhythmia and electrophysiology

Article Title: A Single Protein Kinase A or Calmodulin Kinase II Site Does Not Control the Cardiac Pacemaker Ca 2+ Clock

doi: 10.1161/CIRCEP.115.003180

Figure Lengend Snippet: In vivo basal heart rate and heart rate responses to ISO or spontaneous activity were not altered in RyR2 mutant mice implanted with ECG and activity-sensing telemeters. A. Representative telemetry ECG traces (at baseline and after ISO injection) from S2814A, S2808A and littermate WT (S2808A (−)) mice, scale bar 200 ms. B. Summary of telemetry-recorded heart rates at baseline and in response to ISO stimulation in S2814A, S2808A and littermate control mice. The numerals indicate the number of mice in each experimental group here and in other figures. * p<0.05, ** p<0.01, *** p<0.001, paired Student‘s t-test, Error bars indicate s.e.m. C. Activity related heart rate change in S2814A and WT (C57) control mice, n=3-6. D. Activity related heart rate changes (see Methods) in S2808A and S2808A (−) littermate control mice, n=4- 7. The error bars indicate s.e.m. in all figures.

Article Snippet: Arbitrary activity counts from the telemetry ECG recording system (Data Sciences International) were binned for analysis, with a bin width of 5 counts (see supplemental methods for detail).

Techniques: In Vivo, Activity Assay, Mutagenesis, Injection, Control

Journal: Circulation. Arrhythmia and electrophysiology

Article Title: A Single Protein Kinase A or Calmodulin Kinase II Site Does Not Control the Cardiac Pacemaker Ca 2+ Clock

doi: 10.1161/CIRCEP.115.003180

Figure Lengend Snippet: In vivo heart rate responses to ISO and activity were not affected by Pln knock out or transgenic replacement with WT Pln. A. Representative telemetry ECG recording traces (at baseline and after ISO injection) from Pln−/− and WT-Pln mice. B. Summary ECG telemetry data at baseline and in response to ISO stimulation in WT-Pln and Pln−/− mice. *** p<0.001, paired Student’s t-test. C. Activity related heart rate changes in Pln−/− and WT-Pln mice, n=4-20. D. SAN cell beating rates at baseline and in response to ISO were not affected by Pln knock out. Summary data for SAN cell beating rates at baseline and in response to increasing ISO concentrations. n=8-14 cells/data point. E. Representative INCX current traces induced by caffeine triggered (20 mM) SR Ca2+ release (indicated by arrow) in SAN cells isolated from WT (C57) (left) or Pln−/− (right) mice. The horizontal bars indicate 0 current level. F. SR Ca2+ content was increased in SAN cells isolated from Pln−/− mice compared to SAN cells from WT (C57) mice (C/F: Coulombs/Farad). Numbers of experimental SAN cells is indicated by numerals. ** p<0.01 baseline vs ISO, + p<0.05, +++ p<0.001, Pln−/− vs WT (C57), unpaired Student’s t-test.

Article Snippet: Arbitrary activity counts from the telemetry ECG recording system (Data Sciences International) were binned for analysis, with a bin width of 5 counts (see supplemental methods for detail).

Techniques: In Vivo, Activity Assay, Knock-Out, Transgenic Assay, Injection, Isolation

Journal: Circulation. Arrhythmia and electrophysiology

Article Title: A Single Protein Kinase A or Calmodulin Kinase II Site Does Not Control the Cardiac Pacemaker Ca 2+ Clock

doi: 10.1161/CIRCEP.115.003180

Figure Lengend Snippet: In vivo basal heart rate and response to ISO or activity were not altered in S16A or T17A mice. A. Representative ECG telemetry recording traces (at baseline and after ISO injection) from T17A (upper), S16A (middle) and S16A (−) (lower) mice. B. Summary data of ECG telemetry recorded heart rate at baseline and in response to ISO stimulation in S16A, T17A and their littermate control Pln−/− background mice (S16A(−) and T17A(−)). The number of mice used in the summary data is indicated by numerals in the corresponding bars. * p<0.05, ** p<0.01, *** p<0.001, paired Student’s t-test, Wilcoxon Signed Rank Test was used for T17A group data. C. Activity related heart rate changes in S16A and WT-Pln control mice, n=3-20. D. Activity related heart rate changes (see Methods) in T17A and WT-Pln control mice, n=4-20.

Article Snippet: Arbitrary activity counts from the telemetry ECG recording system (Data Sciences International) were binned for analysis, with a bin width of 5 counts (see supplemental methods for detail).

Techniques: In Vivo, Activity Assay, Injection, Control

Journal: Circulation. Arrhythmia and electrophysiology

Article Title: A Single Protein Kinase A or Calmodulin Kinase II Site Does Not Control the Cardiac Pacemaker Ca 2+ Clock

doi: 10.1161/CIRCEP.115.003180

Figure Lengend Snippet: Reduced in vivo and SAN cell rates in N27A mutant mice. A. Example telemetry ECG recordings (at baseline and after ISO injection) from N27A mutant and WT-Pln mice, scale bar indicates 200 ms. B. Summary data for ECG telemetry recorded heart rates at baseline and in response to ISO stimulation in WT-Pln, N27A and littermate control Pln−/− mice. The number of mice used is indicated in the corresponding bars. *** p<0.001, baseline vs. after ISO, paired Student’s t-test. +++ p<0.001, N27A vs. WT-Pln or Pln−/−, ANOVA, holm-sidak test. C. Activity related heart rate changes in N27A and Pln−/− and WT-Pln control mice, n=4-20. D. SAN cells rates at baseline and in response to ISO were significantly reduced in SAN cells isolated from N27A mice. Example action potential recording traces (at baseline and after ISO application) from SAN cells isolated from WT-Pln (upper), Pln−/− (middle) and N27A (lower) mice. The horizontal bars mark 0 mV. E. Summary data for rates at baseline and in response to increasing ISO concentrations from SAN cells isolated from N27A, Pln−/− and WT-Pln mice. n=6-14 cells/data point. F. Reduced SR Ca2+ content at baseline and after ISO stimulation in SAN cells isolated from N27A mice. The summary SR Ca2+ content data at baseline and after ISO stimulation in SAN cells from WT (C57) and N27A mice. The number of SAN cells studied is indicated by numerals in the corresponding bars. The data for WT (C57) presented here are the same data in shown in Figure 3F. ** p<0.01 baseline vs ISO, +++ p<0.001, N27A vs WT, unpaired Student’s t-test.

Article Snippet: Arbitrary activity counts from the telemetry ECG recording system (Data Sciences International) were binned for analysis, with a bin width of 5 counts (see supplemental methods for detail).

Techniques: In Vivo, Mutagenesis, Injection, Control, Activity Assay, Isolation

Journal: Cardiovascular Research

Article Title: Impaired local regulation of ryanodine receptor type 2 by protein phosphatase 1 promotes atrial fibrillation

doi: 10.1093/cvr/cvu123

Figure Lengend Snippet: Impaired regulation of RyR2 by PP1 increased AF susceptibility in mice. (A) Representative simultaneous recordings of surface ECG and intracardiac electrograms (atrial and ventricular) after burst pacing. (B) Bar graph summarizing the incidence of pacing-induced AF in WT, Sp−/−, and Sp−/−:S2814A mice. Numbers in the bars indicate the number of mice studied. *P < 0.05 vs. WT. #P < 0.05 vs. Sp−/−.

Article Snippet: Telemetry ECG recordings To record ECG from conscious unrestrained mice aged 2–4 months, telemeters (Data Sciences International, MN, USA) were implanted intraperitoneally as previously described.

Techniques:

Journal: Cardiovascular Research

Article Title: Impaired local regulation of ryanodine receptor type 2 by protein phosphatase 1 promotes atrial fibrillation

doi: 10.1093/cvr/cvu123

Figure Lengend Snippet: Impaired regulation of RyR2 by PP1 increased atrial ectopy in mice. (A) Representative telemetry ECG recordings of conscious, freely roaming mice. Arrow points to an atrial ectopic beat in a Sp−/− mouse. (B) Bar graph summarizing the number of atrial ectopic beats per hour in WT, Sp−/−, and Sp−/−:S2814A mice. Numbers under the bars indicate the number of mice studied. *P < 0.05 vs. WT. #P < 0.05 vs. Sp−/−.

Article Snippet: Telemetry ECG recordings To record ECG from conscious unrestrained mice aged 2–4 months, telemeters (Data Sciences International, MN, USA) were implanted intraperitoneally as previously described.

Techniques: